How It Works

Quantibody® employs a matched pair of antibodies for protein biomarker detection. A panel of capture antibodies is printed on a glass slide. After a blocking step, samples are incubated on the arrays. Proteins specific to the capture antibodies are bound to the slide, and the nonspecific proteins are then washed off. The arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluorescence marker. This creates green-fluorescent signals which are visualized using a fluorescence laser scanner. The intensity of the fluorescent signals indicates the amount of the protein biomarkers on the glass slide array. This process of measuring specific amounts of protein molecules is called Quantitative Proteomics.