Nucleic Acid Saliva Collection Kit

Kit Contents

• (10) Saliva Collector
• (1mL) One-step Nucleic Acid Stabilization Buffer
• (10) Microcentrifuge tubes

Additional Materials Required

• Table-top centrifuge
• Precision pipettes

Instructions For Use

1. Before collecting saliva, drink 4 ounces of cold water. Avoid brushing, flossing, or using any other oral hygiene products (e.g. using mouthwash, teeth whitening strips, etc.) for 45 minutes prior to saliva collection.
2. Wait 10 minutes.
3. Spit into the Saliva Collector. Required volume should be determined by the researcher and the intended downstream applications. A minimum volume of 100 μL saliva is suggested.
4. Mix the collected saliva at a 1:1 ratio with the One-Step Nucleic Acid Stabilization Buffer in the provided microcentrifuge tube.
5. Collected sample can be stored at 4ºC for up to 8 hours before use or freeze at -20ºC for long term storage.
6. When ready to analyze, centrifuge the sample using a benchtop centrifuge for 30s to pellet the debris. Pipet the supernatant without disturbing the pellet and continue the assay with the supernatant.